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MethodsIn accordance with a protocol developed a priori, we identified all relevant articles regardless of language by searching Medline (1966 to August 2012), PubMed (2008 to August 2012), Embase (1980 to August 2012), CINAHL (1981 to August 2012), the Cochrane database of systematic reviews, and the Cochrane database of registered clinical trials. We used search strategies recommended for systematic reviews of observational studies.4 We also scanned the bibliographies of identified articles. To allow a systematic review of all studies assessing the association between serum 25 OHD levels and pregnancy outcomes or birth variables our initial search was not limited to observational studies.